Whole-Genome Sequencing of Sordaria macrospora Mutants Identifies Developmental Genes.

ABSTRACT: The study of mutants to elucidate gene functions has a long and successful history; however, to discover causative mutations in mutants that were generated by random mutagenesis often takes years of laboratory work and requires previously generated genetic and/or physical markers, or resources like DNA libraries for complementation. Here, we present an alternative method to identify defective genes in developmental mutants of the filamentous fungus Sordaria macrospora through Illumina/Solexa whole-genome sequencing. We sequenced pooled DNA from progeny of crosses of three mutants and the wild type and were able to pinpoint the causative mutations in the mutant strains through bioinformatics analysis. One mutant is a spore color mutant, and the mutated gene encodes a melanin biosynthesis en

SUBMITTER: Nowrousian M 

PROVIDER: S-EPMC3284333 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature