A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures.

ABSTRACT:

Background

During the routine laboratory cultivation of Lawsonia intracellularis, Mycoplasma contamination has been a frequent problem. When Mycoplasma contamination occurs in laboratories that study L. intracellularis, the cultures must be discarded for 4 reasons: 1) Mycoplasma is inevitably concentrated along with L. intracellularis during the passage of L. intracellularis; 2) Mycoplasma inhibits the growth of L. intracellularis; and 3) it is impossible to selectively eliminate Mycoplasma in L. intracellularis cultures. In this study, we observed the contamination of Mycoplasma species during L. intracellularis cultivation among multiple laboratories.

Results

The presence of a Mycoplasma infection in the L. intracellularis cultures was verified using polymerase chain react

SUBMITTER: Hwang JM 

PROVIDER: S-EPMC3284386 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature