An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars.

ABSTRACT:

Background

Rice genome sequencing projects have generated remarkable amount of information about genes and genome architecture having tremendous potential to be utilized in both basic and applied research. Success in transgenics is paving the way for preparing a road map of functional genomics which is expected to correlate action of a gene to a trait in cellular and organismal context. However, the lack of a simple and efficient method for transformation and regeneration is a major constraint for such studies in this important cereal crop.

Results

In the present study, we have developed an easy, rapid and highly efficient transformation and regeneration protocol using mature seeds as explants and found its successful applicability to a choice of elite indica rice genotypes.

SUBMITTER: Sahoo KK 

PROVIDER: S-EPMC3284416 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature