MicroRNAs targeting oncogenes are down-regulated in pancreatic malignant transformation from benign tumors.

ABSTRACT:

Background

MicroRNA (miRNA) expression profiles have been described in pancreatic ductal adenocarcinoma (PDAC), but these have not been compared with pre-malignant pancreatic tumors. We wished to compare the miRNA expression signatures in pancreatic benign cystic tumors (BCT) of low and high malignant potential with PDAC, in order to identify miRNAs deregulated during PDAC development. The mechanistic consequences of miRNA dysregulation were further evaluated.

Methods

Tissue samples were obtained at a tertiary pancreatic unit from individuals with BCT and PDAC. MiRNA profiling was performed using a custom microarray and results were validated using RT-qPCR prior to evaluation of miRNA targets.

Results

Widespread miRNA down-regulation was observed in PDAC compared to l

SUBMITTER: Jiao LR 

PROVIDER: S-EPMC3284550 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature