De novo identification of viral pathogens from cell culture hologenomes.

ABSTRACT:

Background

Fast, specific identification and surveillance of pathogens is the cornerstone of any outbreak response system, especially in the case of emerging infectious diseases and viral epidemics. This process is generally tedious and time-consuming thus making it ineffective in traditional settings. The added complexity in these situations is the non-availability of pure isolates of pathogens as they are present as mixed genomes or hologenomes. Next-generation sequencing approaches offer an attractive solution in this scenario as it provides adequate depth of sequencing at fast and affordable costs, apart from making it possible to decipher complex interactions between genomes at a scale that was not possible before. The widespread application of next-generation sequencing in th

SUBMITTER: Patowary A 

PROVIDER: S-EPMC3284880 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature