Yeast-based assay identifies novel Shh/Gli target genes in vertebrate development.

ABSTRACT:

Background

The increasing number of developmental events and molecular mechanisms associated with the Hedgehog (Hh) pathway from Drosophila to vertebrates, suggest that gene regulation is crucial for diverse cellular responses, including target genes not yet described. Although several high-throughput, genome-wide approaches have yielded information at the genomic, transcriptional and proteomic levels, the specificity of Gli binding sites related to direct target gene activation still remain elusive. This study aims to identify novel putative targets of Gli transcription factors through a protein-DNA binding assay using yeast, and validating a subset of targets both in-vitro and in-vivo. Testing in different Hh/Gli gain- and loss-of-function scenarios we here identified known (e.g.

SUBMITTER: Milla LA 

PROVIDER: S-EPMC3285088 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature