Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca.

ABSTRACT:

Background

Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project) will fuel the use of genomic tools for studies on parasitic plants. A reliable system for holoparasite transformation is needed to realize the full value of this resource for reverse genetics and functional genomics studies.

Results

Here we demonstrate that transformation of Phelipanche aegyptiaca is achieved by in

SUBMITTER: Fernandez-Aparicio M 

PROVIDER: S-EPMC3285091 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature