Primary role for endoplasmic reticulum-bound ribosomes in cellular translation identified by ribosome profiling.

ABSTRACT: In eukaryotic cells, the spatial regulation of protein expression is frequently conferred through the coupling of mRNA localization and the local control of translation. mRNA localization to the endoplasmic reticulum (ER) is a prominent example of such regulation and serves a ubiquitous role in segregating the synthesis of secretory and integral membrane proteins to the ER. Recent genomic and biochemical studies have now expanded this view to suggest a more substantial role for the ER cellular protein synthesis. We have utilized cell fractionation and ribosome profiling to obtain a genomic survey of the subcellular organization of mRNA translation and report that ribosomal loading of mRNAs, a proxy for mRNA translation, is biased to the ER. Notably, ER-associated mRNAs encoding both cytoso

SUBMITTER: Reid DW 

PROVIDER: S-EPMC3285328 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature