Gene-expression analysis of early- and late-maturation-stage rat enamel organ.

ABSTRACT: Enamel maturation is a dynamic process that involves high rates of mineral acquisition, associated fluctuations in extracellular pH, and resorption of extracellular enamel proteins. During maturation, ameloblasts change from having a tall, thin, and highly polarized organization, characteristic of the secretory stage, to having a low columnar and widened morphology in the maturation stage. To identify potential differences in gene expression throughout maturation, we obtained enamel organ epithelial cells derived from the early- and late-maturation stages of rat incisor and analyzed the global gene-expression profiles at each stage. Sixty-three candidate genes were identified as having potential roles in the maturation process. Quantitative PCR was used to confirm the results of this genom

SUBMITTER: Lacruz RS 

PROVIDER: S-EPMC3286129 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature