Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum).

ABSTRACT:

Background and aims

To date, most floral nectarins (nectar proteins) are reported to function in nectar defence, particularly for insect-pollinated outcrossing species. We compared nectarin composition and abundance in selfing common tobacco (Nicotiana tobaccum) with outcrossing ornamental tobacco plants to elucidate the functional difference of nectarins in different reproductive systems.

Methods

Common tobacco (CT) nectarins were separated by SDS-PAGE and the N terminus of the most abundant nectarin was sequenced via Edman degradation. The full-length nectarin gene was amplified and cloned from genomic DNA and mRNA with hiTail-PCR and RACE (rapid amplification of cDNA ends), and expression patterns were then investigated in different tissues using semi-quantitative reverse

SUBMITTER: Zha HG 

PROVIDER: S-EPMC3286286 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature