Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import.

ABSTRACT:

Background

Integration of human immunodeficiency virus type 1 (HIV-1) into a host cell chromosome is an essential step under the control of the viral integrase (IN). Although this enzyme is necessary and sufficient to catalyze the integration reaction in vitro, cellular cofactors are involved in the process in vivo. The chromatin-associated factor LEDGF/p75 interacts with IN and promotes integration to transcription units of the host genome. HIV-1 IN also binds the karyopherin TNPO3, however the significance of this interaction during viral replication remains to be explored.

Results

Here we present a functional analysis of IN mutants impaired for LEDGF/p75 and TNPO3 interaction. Among them, IN W131A and IN Q168L, that were previously identified to be deficient for LEDGF/p75

SUBMITTER: Cribier A 

PROVIDER: S-EPMC3286403 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature