Comparison of functional proteomic analyses of human breast cancer cell lines T47D and MCF7.

ABSTRACT: T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis

SUBMITTER: Aka JA 

PROVIDER: S-EPMC3286449 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature