Matrix architecture defines the preferential localization and migration of T cells into the stroma of human lung tumors.

ABSTRACT: Appropriate localization and migration of T cells is a prerequisite for antitumor immune surveillance. Studies using fixed tumor samples from human patients have shown that T cells accumulate more efficiently in the stroma than in tumor islets, but the mechanisms by which this occurs are unknown. By combining immunostaining and real-time imaging in viable slices of human lung tumors, we revealed that the density and the orientation of the stromal extracellular matrix likely play key roles in controlling the migration of T cells. Active T cell motility, dependent on chemokines but not on β1 or β2 integrins, was observed in loose fibronectin and collagen regions, whereas T cells migrated poorly in dense matrix areas. Aligned fibers in perivascular regions and around tumor epithelial cell reg

SUBMITTER: Salmon H 

PROVIDER: S-EPMC3287213 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature