Microarray test results should not be compensated for multiplicity of gene contents.

ABSTRACT:

Background

Microarray technology has enabled the measurement of comprehensive transcriptomic information. However, each data entry may reflect trivial individual differences among samples and also contain technical noise. Therefore, the certainty of each observed difference should be confirmed at earlier steps of the analyses, and statistical tests are frequently used for this purpose. Since microarrays analyze a huge number of genes simultaneously, concerns of multiplicity, i.e. the family wise error rate (FWER) and false discovery rate (FDR), have been raised in testing the data. To deal with these concerns, several compensation methodologies have been proposed, making the tests very conservative to the extent that arbitrary tuning of the threshold has been introduced to relax th

SUBMITTER: Konishi T 

PROVIDER: S-EPMC3287486 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature