Revealing the missing expressed genes beyond the human reference genome by RNA-Seq.

ABSTRACT:

Background

The complete and accurate human reference genome is important for functional genomics researches. Therefore, the incomplete reference genome and individual specific sequences have significant effects on various studies.

Results

we used two RNA-Seq datasets from human brain tissues and 10 mixed cell lines to investigate the completeness of human reference genome. First, we demonstrated that in previously identified ~5 Mb Asian and ~5 Mb African novel sequences that are absent from the human reference genome of NCBI build 36, ~211 kb and ~201 kb of them could be transcribed, respectively. Our results suggest that many of those transcribed regions are not specific to Asian and African, but also present in Caucasian. Then, we found that the expressions of 104 RefSeq g

SUBMITTER: Chen G 

PROVIDER: S-EPMC3288009 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature