Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development.

ABSTRACT: Transcription factors have diverse roles during embryonic development, combinatorially controlling cellular states in a spatially and temporally defined manner. Resolving the dynamic transcriptional profiles that underlie these patterning processes is essential for understanding embryogenesis at the molecular level. Here we show how temporal, tissue-specific changes in embryonic transcription factor function can be discerned by integrating caged morpholino oligonucleotides with photoactivatable fluorophores, fluorescence-activated cell sorting and microarray technologies. As a proof of principle, we have dynamically profiled No tail a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, discovering discrete sets of transcripts that are coincident with eith

SUBMITTER: Shestopalov IA 

PROVIDER: S-EPMC3288381 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature