Molecular responses of choroidal endothelial cells to elastin derived peptides through the elastin-binding protein (GLB1).

ABSTRACT:

Purpose

Neovascular AMD involves the activation of choroidal endothelial cells to increase their inflammatory and angiogenic behaviors. Elastin derived peptides (EDPs) can elicit some of these phenotypic changes in endothelial cells. This investigation was performed to follow up on those findings by determining a receptor for these peptides in the human eye as well as evaluating the effects of elevated EDPs on choroidal cells in vitro and in vivo.

Methods

The expression of elastin receptor genes including GLB1 was analyzed using reverse transcription PCR. Migration of choroidal endothelial cells was quantified in the presence of inhibitors to different EDP binding proteins. C57BL6 mice were injected with EDPs and studied by electroretinography, transmission electron microsco

SUBMITTER: Skeie JM 

PROVIDER: S-EPMC3288713 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature