Matriptase protects against experimental colitis and promotes intestinal barrier recovery.

ABSTRACT:

Background

Matriptase is a membrane-anchored serine protease encoded by suppression of tumorigenicity-14 (ST14) that is required for epithelial barrier homeostasis. However, its functional role in inflammatory bowel disease (IBD) is unexplored.

Methods

Matriptase expression in control, Crohn's disease, and ulcerative colitis tissue specimens was studied by quantitative polymerase chain reaction (qPCR) and immunostaining. Matriptase function was investigated by subjecting St14 hypomorphic and control littermates to dextran sodium sulfate (DSS)-induced colitis and by siRNA silencing in cultured monolayers. Mice were analyzed for clinical, histological, molecular, and cellular effects.

Results

Matriptase protein and ST14 mRNA levels are significantly downregulated in inf

SUBMITTER: Netzel-Arnett S 

PROVIDER: S-EPMC3288858 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature