Left-right symmetry breaking in tissue morphogenesis via cytoskeletal mechanics.

ABSTRACT:

Rationale

Left-right (LR) asymmetry is ubiquitous in animal development. Cytoskeletal chirality was recently reported to specify LR asymmetry in embryogenesis, suggesting that LR asymmetry in tissue morphogenesis is coordinated by single- or multi-cell organizers. Thus, to organize LR asymmetry at multiscale levels of morphogenesis, cells with chirality must also be present in adequate numbers. However, observation of LR asymmetry is rarely reported in cultured cells.

Objectives

Using cultured vascular mesenchymal cells, we tested whether LR asymmetry occurs at the single cell level and in self-organized multicellular structures.

Methods and results

Using micropatterning, immunofluorescence revealed that adult vascular cells polarized rightward and accumulated stress

SUBMITTER: Chen TH 

PROVIDER: S-EPMC3288887 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature