Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach.

ABSTRACT: Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing approaches for species with large complex genomes such as barley and wheat. Genotyping-by-sequencing (GBS) has been developed as a tool for association studies and genomics-assisted breeding in a range of species including those with complex genomes. GBS uses restriction enzymes for targeted complexity reduction followed by multiplex sequencing to produce high-quality polymorphism data at a relatively low per sample cost. Here we present a GBS approach for species that currently lack a reference genome sequence. We developed a novel two-en

SUBMITTER: Poland JA 

PROVIDER: S-EPMC3289635 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature