Increased expression of Syne1/nesprin-1 facilitates nuclear envelope structure changes in embryonic stem cell differentiation.

ABSTRACT: We found by electron microscopy that the inter-membrane space of embryonic stem cells is irregular and generally wider than in differentiated cells. Among a panel of nuclear envelope structural proteins examined, the expression of Syne1/nesprin-1 was found to be greatly induced upon differentiation. Down-regulation of Syne1 by siRNA in differentiated embryonic stem cells caused the nuclear envelope to adopt a configuration resembling that found in undifferentiated embryonic stem cells. Suppression of Syne1 expression did not produce a detectable impact on the retinoic acid-induced differentiation of embryonic stem cells; however, forced expression of Syne1 enhanced the tendency of the cells to lose pluripotency. Thus, we found that low expression of Syne1 splicing isoforms accounts for the

SUBMITTER: Smith ER 

PROVIDER: S-EPMC3290128 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature