Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation.

ABSTRACT: Mitotic spindle positioning by cortical pulling forces defines the cell division axis and location, which is critical for proper cell division and development. Although recent work has identified developmental and extrinsic cues that regulate spindle orientation, the contribution of intrinsic signals to spindle positioning and orientation remains unclear. Here, we demonstrate that cortical force generation in human cells is controlled by distinct spindle-pole- and chromosome-derived signals that regulate cytoplasmic dynein localization. First, dynein exhibits a dynamic asymmetric cortical localization that is negatively regulated by spindle-pole proximity, resulting in spindle oscillations to centre the spindle within the cell. We find that this signal comprises the spindle-pole-localized

SUBMITTER: Kiyomitsu T 

PROVIDER: S-EPMC3290711 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature