Requirement of myeloid cell-specific Fas expression for prevention of systemic autoimmunity in mice.

ABSTRACT:

Objective

The death receptor Fas is a critical mediator of the extrinsic apoptotic pathway, and its role in mediating lymphoproliferation has been extensively examined. The present study was undertaken to investigate the impact of myeloid cell-specific loss of Fas.

Methods

Mice with Fas flanked by loxP sites (Fas(flox/flox) ) were crossed with mice expressing Cre under control of the murine lysozyme M gene promoter (Cre(LysM) ), which functions in mature lysozyme-expressing cells of the myelomonocytic lineage. The genotype for Cre(LysM) Fas(flox/flox) mice was verified by polymerase chain reaction and flow cytometric analysis. Flow cytometric analysis was also used to characterize myeloid, dendritic, and lymphoid cell distribution and activation in bone marrow, blood, and s

SUBMITTER: Cuda CM 

PROVIDER: S-EPMC3290732 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature