Interaction between methyl CpG-binding protein and ran GTPase during cell division in tobacco cultured cells.

ABSTRACT:

Background and aims

Methyl CpG-binding proteins are considered to play critical roles in epigenetic control of gene expression by recognizing and interacting with 5-methylcytosine (m(5)C) in eukaryotes. However, among 13 corresponding genes in Arabidopsis thaliana, designated as featuring a methyl-binding domain (MBD), only four have so far been shown actually to bind to m(5)C. One example, AtMBD5, was selected here to screen for interacting proteins.

Methods

Yeast two-hybrid assays were used for screening, and physical interaction was confirmed by pull-down and bimolecular fluorescence complementation (BiFC) assays. Cellular localization was analysed by fluorescence-tagged fusion proteins using tobacco (Nicotiana tabacum) cultured bright yellow 2 cells.

Key results

A

SUBMITTER: Yano A 

PROVIDER: S-EPMC3292272 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature