Generation of stable pluripotent stem cells from NOD mouse tail-tip fibroblasts.

ABSTRACT:

Objective

The NOD mouse strain has been widely used to investigate the pathology and genetic susceptibility for type 1 diabetes. Induced pluripotent stem cells (iPSCs) derived from this unique mouse strain would enable new strategies for investigating type 1 diabetes pathogenesis and potential therapeutic targets. The objective of this study was to determine whether somatic fibroblasts from NOD mice could be reprogrammed to become iPSCs, providing an alternative source of stem cells for the production of genetically modified NOD cells and mice.

Research design and methods

Adult tail-tip fibroblasts from male NOD mice were reprogrammed by retroviral transduction of the coding sequences of three transcription factors, OCT4, SOX2, and KLF4, in combination with a histone deacety

SUBMITTER: Liu J 

PROVIDER: S-EPMC3292312 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature