Validation of a set of reference genes to study response to herbicide stress in grasses.

ABSTRACT:

Background

Non-target-site based resistance to herbicides is a major threat to the chemical control of agronomically noxious weeds. This adaptive trait is endowed by differences in the expression of a number of genes in plants that are resistant or sensitive to herbicides. Quantification of the expression of such genes requires normalising qPCR data using reference genes with stable expression in the system studied as internal standards. The aim of this study was to validate reference genes in Alopecurus myosuroides, a grass (Poaceae) weed of economic and agronomic importance with no genomic resources.

Results

The stability of 11 candidate reference genes was assessed in plants resistant or sensitive to herbicides subjected or not to herbicide stress using the complementary

SUBMITTER: Petit C 

PROVIDER: S-EPMC3292489 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature