Dispensability of APRIL to the development of systemic lupus erythematosus in NZM 2328 mice.

ABSTRACT:

Objective

To determine the role of APRIL in the development of systemic lupus erythematosus (SLE) in mice.

Methods

Wild-type (WT) NZM 2328, NZM. April(-/-) , NZM.Baff(-/-) , and NZM.Baff(-/-) .April(-/-) mice were evaluated for lymphocyte phenotype by flow cytometry, for serum total IgG and IgG autoantibody levels by enzyme-linked immunosorbent assay, for glomerular deposition of IgG and C3 by immunofluorescence, for renal changes by histopathology, and for clinical disease by laboratory assessment (severe proteinuria).

Results

In comparison to WT mice, NZM.April(-/-) mice harbored increased spleen B cells, T cells, and plasma cells (PCs), increased serum levels of IgG antichromatin antibodies, and decreased numbers of bone marrow (BM) PCs. Glomerular deposition of

SUBMITTER: Jacob CO 

PROVIDER: S-EPMC3292707 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature