Methodology optimizing SAGE library tag-to-gene mapping: application to Leishmania.

ABSTRACT:

Background

Leishmaniasis are widespread parasitic-diseases with an urgent need for more active and less toxic drugs and for effective vaccines. Understanding the biology of the parasite especially in the context of host parasite interaction is a crucial step towards such improvements in therapy and control. Several experimental approaches including SAGE (Serial analysis of gene expression) have been developed in order to investigate the parasite transcriptome organisation and plasticity. Usual SAGE tag-to-gene mapping techniques are inadequate because almost all tags are normally located in the 3'-UTR outside the CDS, whereas most information available for Leishmania transcripts is restricted to the CDS predictions. The aim of this work is to optimize a SAGE libraries tag-to-gene m

SUBMITTER: Smandi S 

PROVIDER: S-EPMC3292834 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature