Analysis of CpG methylation sites and CGI among human papillomavirus DNA genomes.

ABSTRACT:

Background

The Human Papillomavirus (HPV) genome is divided into early and late coding sequences, including 8 open reading frames (ORFs) and a regulatory region (LCR). Viral gene expression may be regulated through epigenetic mechanisms, including cytosine methylation at CpG dinucleotides. We have analyzed the distribution of CpG sites and CpG islands/clusters (CGI) among 92 different HPV genomes grouped in function of their preferential tropism: cutaneous or mucosal. We calculated the proportion of CpG sites (PCS) for each ORF and calculated the expected CpG values for each viral type.

Results

CpGs are underrepresented in viral genomes. We found a positive correlation between CpG observed and expected values, with mucosal high-risk (HR) virus types showing the smallest O/E

SUBMITTER: Galvan SC 

PROVIDER: S-EPMC3293833 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature