Francisella RNA polymerase contains a heterodimer of non-identical α subunits.

ABSTRACT:

Background

All sequenced genomes of representatives of the Francisella genus contain two rpoA genes, which encode non-identical RNA polymerase (RNAP) subunits, α1 and α2. In all other bacteria studied to date, a dimer of identical α subunits initiates the assembly of the catalytically proficient RNAP core (subunit composition α2ββ'). Based on an observation that both α1 and α2 are incorporated into Francisella RNAP, Charity et al. (2007) previously suggested that up to four different species of RNAP core enzyme might form in the same Francisella cell.

Results

By in vitro assembly from fully denatured state, we determined that both Francisella α subunits are required for efficient dimerization; no homodimer formation was detected. Bacterial two-hybrid system analysis likewise

SUBMITTER: Mukhamedyarov D 

PROVIDER: S-EPMC3294249 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature