Use of the chinchilla model for nasopharyngeal colonization to study gene expression by Moraxella catarrhalis.

ABSTRACT: Young adult chinchillas were atraumatically inoculated with Moraxella catarrhalis via the nasal route. Detailed histopathologic examination of nasopharyngeal tissues isolated from these M. catarrhalis-infected animals revealed the presence of significant inflammation within the epithelium. Absence of similar histopathologic findings in sham-inoculated animals confirmed that M. catarrhalis was exposed to significant host-derived factors in this environment. Twenty-four hours after inoculation, viable M. catarrhalis organisms were recovered from the nasal cavity and nasopharynx of the animals in numbers sufficient for DNA microarray analysis. More than 100 M. catarrhalis genes were upregulated in vivo, including open reading frames (ORFs) encoding proteins that are involved in a truncated de

SUBMITTER: Hoopman TC 

PROVIDER: S-EPMC3294655 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature