SoxS increases the expression of the zinc uptake system ZnuACB in an Escherichia coli murine pyelonephritis model.

ABSTRACT: Paralogous transcriptional regulators MarA, Rob, and SoxS act individually and together to control expression of more than 80 Escherichia coli genes. Deletion of marA, rob, and soxS from an E. coli clinical isolate prevents persistence beyond 2 days postinfection in a mouse model of pyelonephritis. We used microarray analysis to identify 242 genes differentially expressed between the triple deletion mutant and its parent strain at 2 days postinfection in the kidney. One of these, znuC of the zinc transport system ZnuACB, displayed decreased expression in the triple mutant compared to that in the parental strain, and deletion of znuC from the parental strain reduced persistence. The marA rob soxS triple deletion mutant was less viable in vitro under limited-Zn and Zn-depleted conditions, wh

SUBMITTER: Warner DM 

PROVIDER: S-EPMC3294818 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature