Impact of a disruption of a pathway delivering copper to mitochondria on Podospora anserina metabolism and life span.

ABSTRACT: A global depletion of cellular copper as the result of a deficiency in high-affinity copper uptake was previously shown to affect the phenotype and life span of the filamentous fungus Podospora anserina. We report here the construction of a strain in which the delivery of copper to complex IV of the mitochondrial respiratory chain is affected. This strain, PaCox17::ble, is a PaCox17-null mutant that does not synthesize the molecular chaperone targeting copper to cytochrome c oxidase subunit II. PaCox17::ble is characterized by a decreased growth rate, a reduction in aerial hyphae formation, reduced female fertility, and a dramatic increase in life span. The mutant respires via a cyanide-resistant alternative pathway, displays superoxide dismutase (SOD) activity profiles significantly diffe

SUBMITTER: Stumpferl SW 

PROVIDER: S-EPMC329504 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature