Development and validation of a comparative genomic fingerprinting method for high-resolution genotyping of Campylobacter jejuni.

ABSTRACT: Campylobacter spp. are a leading cause of bacterial gastroenteritis worldwide. The need for molecular subtyping methods with enhanced discrimination in the context of surveillance- and outbreak-based epidemiologic investigations of Campylobacter spp. is critical to our understanding of sources and routes of transmission and the development of mitigation strategies to reduce the incidence of campylobacteriosis. We describe the development and validation of a rapid and high-resolution comparative genomic fingerprinting (CGF) method for C. jejuni. A total of 412 isolates from agricultural, environmental, retail, and human clinical sources obtained from the Canadian national integrated enteric pathogen surveillance program (C-EnterNet) were analyzed using a 40-gene assay (CGF40) and multilocus

SUBMITTER: Taboada EN 

PROVIDER: S-EPMC3295178 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature