Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering.

ABSTRACT: Repeat proteins have recently been of great interest as potential alternatives to immunoglobulin antibodies due to their unique structural and biophysical features. We here present the development of a binding scaffold based on variable lymphocyte receptors, which are nonimmunoglobulin antibodies composed of Leucine-rich repeat modules in jawless vertebrates, by module engineering. A template scaffold was first constructed by joining consensus repeat modules between the N- and C-capping motifs of variable lymphocyte receptors. The N-terminal domain of the template scaffold was redesigned based on the internalin-B cap by analyzing the modular similarity between the respective repeat units using a computational approach. The newly designed scaffold, termed "Repebody," showed a high level of

SUBMITTER: Lee SC 

PROVIDER: S-EPMC3295290 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature