Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway.

ABSTRACT:

Unlabelled

ΒACKGROUND: The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a sole carbon source. The methanol utilization pathway describes all the catalytic reactions, which happen during methanol metabolism. Despite the importance of certain key enzymes in this pathway, so far very little is known about possible effects of overexpressing either of these key enzymes on the overall energetic behavior, the productivity and the substrate uptake rate in P. pastoris strains.

Results

A fast and easy-to-do approach based on batch cultivations with methanol pulses was used to characterize different P. pastoris strains. A strain with MutS phenotype was found to be superior over a strain with

SUBMITTER: Krainer FW 

PROVIDER: S-EPMC3295664 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature