Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells.

ABSTRACT: Significant efforts to develop both laboratory and field-based detection assays for an array of potential biological threats started well before the anthrax attacks of 2001 and have continued with renewed urgency following. While numerous assays and methods have been explored that are suitable for laboratory utilization, detection in the field is often complicated by requirements for functionality in austere environments, where limited cold-chain facilities exist. In an effort to overcome these assay limitations for Bacillus anthracis, one of the most recognizable threats, a series of single domain antibodies (sdAbs) were isolated from a phage display library prepared from immunized llamas. Characterization of target specificity, affinity, and thermal stability was conducted for six sdAb f

SUBMITTER: Walper SA 

PROVIDER: S-EPMC3295763 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature