High resolution profiling of human exon methylation by liquid hybridization capture-based bisulfite sequencing.

ABSTRACT:

Background

DNA methylation plays important roles in gene regulation during both normal developmental and disease states. In the past decade, a number of methods have been developed and applied to characterize the genome-wide distribution of DNA methylation. Most of these methods endeavored to screen whole genome and turned to be enormously costly and time consuming for studies of the complex mammalian genome. Thus, they are not practical for researchers to study multiple clinical samples in biomarker research.

Results

Here, we display a novel strategy that relies on the selective capture of target regions by liquid hybridization followed by bisulfite conversion and deep sequencing, which is referred to as liquid hybridization capture-based bisulfite sequencing (LHC-BS). To e

SUBMITTER: Wang J 

PROVIDER: S-EPMC3295804 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature