Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps.
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ABSTRACT:
SUBMITTER: Verchere A
PROVIDER: S-EPMC3296093 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps.
Verchère Alice A Broutin Isabelle I Picard Martin M
Scientific reports 20120307
We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump. ...[more]
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