Antisense sequencing improves the accuracy and precision of A-to-I editing measurements using the peak height ratio method.

ABSTRACT:

Background

A-to-I RNA editing is found in all phyla of animals and contributes to transcript diversity that may have profound impacts on behavior and physiology. Many transcripts of genes involved in axonal conductance, synaptic transmission and modulation are the targets of A-to-I RNA editing. There are a number of methods to measure the extent of A-to-I RNA editing, but they are generally costly and time consuming. One way to determine the frequency of A-to-I RNA editing is the peak height ratio method, which compares the size of peaks on electropherograms that represent unedited and edited sites.

Findings

Sequencing of 4 editing sites of the Dα6 nicotinic acetylcholine receptor subunit with an antisense primer (which uses T/C peaks to measure unedited and edited sites, re

SUBMITTER: Rinkevich FD 

PROVIDER: S-EPMC3296654 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature