Analysis of the salivary microbiome using culture-independent techniques.

ABSTRACT:

Background

The salivary microbiota is a potential diagnostic indicator of several diseases. Culture-independent techniques are required to study the salivary microbial community since many of its members have not been cultivated.

Methods

We explored the bacterial community composition in the saliva sample using metagenomic whole genome shotgun (WGS) sequencing, the extraction of 16S rRNA gene fragments from metagenomic sequences (16S-WGS) and high-throughput sequencing of PCR-amplified bacterial 16S rDNA gene (16S-HTS) regions V1 and V3.

Results

The hierarchical clustering of data based on the relative abundance of bacterial genera revealed that distances between 16S-HTS datasets for V1 and V3 regions were greater than those obtained for the same V region with differe

SUBMITTER: Lazarevic V 

PROVIDER: S-EPMC3296672 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature