Expression divergence measured by transcriptome sequencing of four yeast species.

ABSTRACT:

Background

The evolution of gene expression is a challenging problem in evolutionary biology, for which accurate, well-calibrated measurements and methods are crucial.

Results

We quantified gene expression with whole-transcriptome sequencing in four diploid, prototrophic strains of Saccharomyces species grown under the same condition to investigate the evolution of gene expression. We found that variation in expression is gene-dependent with large variations in each gene's expression between replicates of the same species. This confounds the identification of genes differentially expressed across species. To address this, we developed a statistical approach to establish significance bounds for inter-species differential expression in RNA-Seq data based on the variance measur

SUBMITTER: Busby MA 

PROVIDER: S-EPMC3296765 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature