Detection of IL28B SNP DNA from buccal epithelial cells, small amounts of serum, and dried blood spots.

ABSTRACT:

Background & aims

Point mutations in the coding region of the interleukin 28 gene (rs12979860) have recently been identified for predicting the outcome of treatment of hepatitis C virus infection. This polymorphism detection was based on whole blood DNA extraction. Alternatively, DNA for genetic diagnosis has been derived from buccal epithelial cells (BEC), dried blood spots (DBS), and genomic DNA from serum. The aim of the study was to investigate the reliability and accuracy of alternative routes of testing for single nucleotide polymorphism allele rs12979860CC.

Methods

Blood, plasma, and sera samples from 200 patients were extracted (400 µL). Buccal smears were tested using an FTA card. To simulate postal delay, we tested the influence of storage at ambient temperature on

SUBMITTER: Halfon P 

PROVIDER: S-EPMC3296767 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature