S100A4 silencing blocks invasive ability of esophageal squamous cell carcinoma cells.

ABSTRACT:

Aim

To investigate a potential role of S100A4 in esophagus squamous cell carcinoma metastasis (ESCCs).

Methods

Expression of S100A4 and E-cadherin were analyzed in frozen sections from ESCCs (metastasis, n = 28; non-metastasis, n = 20) by reverse transcription-polymerase chain reaction, quantitative polymerase chain reaction and immunohistochemistry. To explore the influence of S100A4 on esophageal cancer invasion and metastasis, S100A4 was overexpressed or silenced by S100A4 siRNA in TE-13 or Eca-109 cells in vitro and in vivo.

Results

We found the mRNA and protein levels of S100A4 expression in ESCCs was significantly upregulated, and more importantly, that expression of S100A4 and E cadherin are strongly negatively correlated in patients who had metastasis. It was

SUBMITTER: Chen D 

PROVIDER: S-EPMC3297050 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature