Response of fatty acid synthesis genes to the binding of human salivary amylase by Streptococcus gordonii.

ABSTRACT: Streptococcus gordonii, an important primary colonizer of dental plaque biofilm, specifically binds to salivary amylase via the surface-associated amylase-binding protein A (AbpA). We hypothesized that a function of amylase binding to S. gordonii may be to modulate the expression of chromosomal genes, which could influence bacterial survival and persistence in the oral cavity. Gene expression profiling by microarray analysis was performed to detect genes in S. gordonii strain CH1 that were differentially expressed in response to the binding of purified human salivary amylase versus exposure to purified heat-denatured amylase. Selected genes found to be differentially expressed were validated by quantitative reverse transcription-PCR (qRT-PCR). Five genes from the fatty acid synthesis (FAS)

SUBMITTER: Nikitkova AE 

PROVIDER: S-EPMC3298122 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature