Hepatitis C virus infection of neuroepithelioma cell lines.

ABSTRACT:

Background & aims

Hepatitis C virus (HCV) establishes chronic infections in 3% of the world's population. Infection leads to progressive liver disease; hepatocytes are the major site of viral replication in vivo. However, chronic infection is associated with a variety of extrahepatic syndromes, including central nervous system (CNS) abnormalities. We therefore screened a series of neural and brain-derived cell lines for their ability to support HCV entry and replication.

Methods

We used a panel of neural-derived cell lines, HCV pseudoparticles (HCVpp), and an infectious, HCV JFH-1 cell-culture system (HCVcc) to assess viral tropism.

Results

Two independently derived neuroepithelioma cell lines (SK-N-MC and SK-PN-DW) permitted HCVpp entry. In contrast, several neurobla

SUBMITTER: Fletcher NF 

PROVIDER: S-EPMC3298458 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature