Inhibition of dendritic cell migration by transforming growth factor-β1 increases tumor-draining lymph node metastasis.

ABSTRACT:

Background

Transforming growth factor (TGF)-β is known to be produced by progressor tumors and to immobilize dendritic cells (DCs) within those tumors. Moreover, although TGF-β1 has been shown to promote tumor progression, there is still no direct, in vivo evidence as to whether TGF-β1 is able to directly induce distant metastasis.

Methods

To address that issue and investigate the mechanism by which TGF-β1 suppresses DC activity, we subdermally inoculated mouse ears with squamous cell carcinoma cells stably expressing TGF-β1 or empty vector (mock).

Results

The numbers of DCs within lymph nodes draining the resultant TGF-β1-expressing tumors was significantly lower than within nodes draining tumors not expressing TGF-β1. We then injected fluorescently labeled bone marr

SUBMITTER: Imai K 

PROVIDER: S-EPMC3298529 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature