Non-genomic effects of PPARgamma ligands: inhibition of GPVI-stimulated platelet activation.

ABSTRACT:

Background

Peroxisome proliferator-activated receptor-(gamma) (PPAR(gamma)) is expressed in human platelets although in the absence of genomic regulation in these cells, its functions are unclear.

Objective

In the present study, we aimed to demonstrate the ability of PPAR(gamma) ligands to modulate collagen-stimulated platelet function and suppress activation of the glycoprotein VI (GPVI) signaling pathway.

Methods

Washed platelets were stimulated with PPAR(gamma) ligands in the presence and absence of PPAR(gamma) antagonist GW9662 and collagen-induced aggregation was measured using optical aggregometry. Calcium levels were measured by spectrofluorimetry in Fura-2AM-loaded platelets and tyrosine phosphorylation levels of receptor-proximal components of the GPVI signal

SUBMITTER: Moraes LA 

PROVIDER: S-EPMC3298645 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature