Common changes in global gene expression induced by RNA polymerase inhibitors in Shigella flexneri.

ABSTRACT: Characterization of expression profile of organisms in response to antimicrobials provides important information on the potential mechanism of action of the drugs. The special expression signature can be used to predict whether other drugs act on the same target. Here, the common response of Shigella flexneri to two inhibitors of RNA polymerase was examined using gene expression profiling. Consistent with similar effects of the two drugs, the gene expression profiles indicated that responses of the bacteria to these drugs were roughly the same, with 225 genes affected commonly. Of them, 88 were induced and 137 were repressed. Real-time PCR was performed for selected genes to verify the microarray results. Analysis of the expression data revealed that more than 30% of the plasmid-encoded ge

SUBMITTER: Fu H 

PROVIDER: S-EPMC3299763 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature