Expression and regulation of the Escherichia coli O157:H7 effector proteins NleH1 and NleH2.

ABSTRACT:

Background

E. coli O157 carries two genes encoding the effector proteins NleH1 and NleH2 which are 87% identical. Despite the similarity between the proteins, the promoter regions upstream of the genes encoding the effectors are more divergent suggesting that the actual expression of the genes may be differentially regulated. This was tested by creating reporter fusions and examining their expression in different genetic backgrounds, media and on contact with host cells. The function of the proteins was also tested following transfection into host cells.

Principal findings

Expression of both NleH1 and NleH2 was enhanced when cultured under conditions that stimulated expression of the Type Three Secretion System (T3SS) and was influenced by the regulators Ler and GrlA. Maxima

SUBMITTER: Holmes A 

PROVIDER: S-EPMC3299786 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature